The Oblate Fathers Community Crèche in Inchicore and the Home from Home Crèche in Greystones, Co. Wicklow, will have to close within 21 days.
Tusla says they're being shut down for 'persistent and serious' breaches of regulations.
As the Dublin crèche is a non-profit, serving a highly disadvantaged area, steps are being taken to potentially keep it running under new management.
Tusla says it acknowledges the impact the closures will have on parents, children and staff, but added both providers were given every opportunity to make improvements.
